Library Detail Page for K326_NtRoot
| Short Name: | K326_NtRoot |
| Organism: | Nicotiana tabacum |
| Library Name: | K326 growing stage roots (tobacco suppress. subtract. hybridiz. cDNA library) |
| Total Sequences: | 405 sequences from 408 clones |
| Average Sequence Length: | 390.9 (Standard deviation 188.9) |
| Type: | cDNA Library |
| Tissue: | |
| Development Stage: | vigorous growing stage |
| Treatment Conditions: | |
| Cloning Host: | DH5a |
| Cloning Kit: | |
| Comments | Organ: Roots, Vector: pGEM-T-Easy, The tobacco suppression subtractive hybridization cDNA library was constructed from tobacco cultivar k326. The root were collected at vigorous growing stage, and placed into liquid N2 immediately and stored in -80oC freezer. Total RNA was isolated using TRNzol-Reagent (Tangen), mRNA was extracted from total RNA by mRNA extraction kit (promega). cDNA synthesis and differentially expressed RNA was prepared following the protocol of CLONTECH PCR-SelectTM cDNA Subtraction kit. Then the subtracted cDNA collection was used to construct library directly with pGEM-T-Easy vector. The library was constructed by Dr. Jianqing Pan and Dr. Weijian Zhuang. |
| Authors | Pan,J.Q., Zhuang,W.J., Lan,L. and Li,W.X. |
| Contact Information |
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