Library Detail Page for LeHSU
| Short Name: | LeHSU |
| Organism: | Solanum lycopersicum (formerly Lycopersicon esculentum) |
| Library Name: | Tomato shoot subtractive cDNA library for heat-shock up-regulated genes |
| Total Sequences: | 122 sequences from 122 clones |
| Average Sequence Length: | 725.2 (Standard deviation 123.9) |
| Type: | cDNA Library |
| Tissue: | shoots |
| Development Stage: | Three weeks old |
| Treatment Conditions: | |
| Cloning Host: | DH5a |
| Cloning Kit: | |
| Comments | Vector: pGEM-Teasy, The subtractive library was created by using mRNA purified from tomato shoots with or without heat-shock treatment. Tomato shoots were harvested from plants of 3 weeks old seedlings grown on MS medium. Plants were heat-treated by incubated at 40 degree C in the dark for 2 h followed by recovery at room temperature for 30 min. Library of up-regulated cDNA was generated by subtracting the heat-treated mRNA with non-treated ones. |
| Authors | Liu,N.Y., Ko,S.S., Yeh,K.C. and Charng,Y.Y. |
| Contact Information |
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