Library Detail Page for LePS
| Short Name: | LePS |
| Organism: | Solanum lycopersicum (formerly Lycopersicon esculentum) |
| Library Name: | Subtractive cDNA library of roots under phosphate starvation |
| Total Sequences: | 817 sequences from 823 clones |
| Average Sequence Length: | 499.1 (Standard deviation 183.8) |
| Type: | cDNA Library |
| Tissue: | root |
| Development Stage: | 5 weeks old plants |
| Treatment Conditions: | |
| Cloning Host: | Top 10 F' |
| Cloning Kit: | |
| Comments | Vector: PGEM Teasy, Tomato plants were grown hydroponically in modified Hoagland's solution containing 0.5 mM of KH2PO4. 4-week-old plants were subjected for phosphate starvation treatment by omitting the KH2PO4 from the nutrient solution. Tomato roots were harvested after 7-day phosphate starvation. Total and poly A+ RNA was isolated from phosphate starvation and control roots, respectively. Subtractive cDNA library was constructed using PCR-Select cDNA subtraction Kit (Clontech) to selectively amplify up-regulated genes under phosphate starvation condition. |
| Authors | Liu,N.Y., Ko,S.S., Yeh,K.C., Charng,Y.Y., Shieh,S.Y., Wang,J.G., Lin,S.I., Cheng,P.W. and Chiou,T.J |
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