Library Detail Page for LeTrich01
| Short Name: | LeTrich01 |
| Organism: | Solanum lycopersicum (formerly Lycopersicon esculentum) |
| Library Name: | Solanum lycopersicum trichomes |
| Total Sequences: | 7159 sequences from 7299 clones |
| Average Sequence Length: | 561.4 (Standard deviation 189.2) |
| Type: | cDNA Library |
| Tissue: | Isolated leaf trichomes |
| Development Stage: | Apical internodes of 5-week old plants. |
| Treatment Conditions: | |
| Cloning Host: | |
| Cloning Kit: | |
| Comments | Vector: pDNR_lib (Clontech), Site_1: Sfi I A (GGCCATTACGGCC), Site_2: Sfi I B (GGCCGCCTCGGCC), Solanum lycopersicum plants were grown on compost in the greenhouse with supplemental lighting (4 h in winter) providing a minimum illumination period of 14 h. Trichomes were harvested from the most apical leaves of 5 week old Solanum lycopersicum plants. Trichomes were harvested by brushing leaves frozen using liquid nitrogen and separated from contaminating leaf fragments by passing the frozen material through a sieve. RNA was isolated from the trichomes using the Qiagen RNeasyMini kit. A cDNA EST library was generated following manufacturers instructions using the Creator SMART cDNA library construction kit (BD Biosciences) starting from total RNA and using the pDNR-LIB vector. |
| Authors | Besser,K., Harper,A., Welsby,N.A., Schauvinhold,I., Slocombe,S.P., Li,Y., Dixon,R.A. and Broun,P. |
| Contact Information |
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