Library Detail Page for SAL_US
Short Name: | SAL_US |
Organism: | Nicotiana benthamiana |
Library Name: | SAL_US N.Bentheme |
Total Sequences: | 8083 sequences from 8088 clones |
Average Sequence Length: | 558.3 (Standard deviation 105.1) |
Type: | cDNA Library |
Tissue: | expanding leaf tissue |
Development Stage: | |
Treatment Conditions: | |
Cloning Host: | |
Cloning Kit: | |
Comments | Vector: pBluescript S/K +, Site_1: EcoR1, Site_2: Xho1, Total RNA isolated from tissue, poly A fraction isolated on oligo-dT column. mRNA reverse transcribed and double stranded. Poly A dsRNA ranging in size from 400-4000 bp directionally cloned into EcoR1 and Xho1 restricted into pBluescript. EcoR1 site at the 5' end of the cDNA and Xh01 on the 3' side. |
Authors | Opperman,C.H., Lommel,S.A,, Burke,M., Feulner,G., Carlson,J., George,C., Gove,S., Houfek,T.D., Jefferys,S.R., Kalat,S., King,R., Levin,J., Little,P.C., Lumpkin,A., Ross,T., Salstead,A., Scholl,E.H., Sosinski,B.R., Stephens,P.J. and Zekanis,S.H. |
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