Library Detail Page for Sl_RootInf02
| Short Name: | Sl_RootInf02 |
| Organism: | Solanum lycopersicum (formerly Lycopersicon esculentum) |
| Library Name: | Forward subtractive cDNA library of tomato inoculated with Ralstonia solanacearu |
| Total Sequences: | 332 sequences from 333 clones |
| Average Sequence Length: | 458.0 (Standard deviation 162.8) |
| Type: | cDNA Library |
| Tissue: | root and collar |
| Development Stage: | Three weeks old |
| Treatment Conditions: | |
| Cloning Host: | XL1-Blue |
| Cloning Kit: | |
| Comments | Vector: pGEM-Teasy, 3 week-old plants (cultivar Hawaii 7996) were inoculated with water (control) or Ralstonia solanacearum (an incompatible strain) (Rs-inoculated). Roots and collars of control and Rs-inoculated plants were harvested 24 and 48 hours post inoculation. Poly(A) RNA specimen prepared from control plant samples were mixed at 1:1 ratio, and that prepared from Rs-inoculated samples were also mixed at 1:1 ratio. Tomato cDNA library was then constructed by subtracting the pooled control specimen from pooled Rs-inoculated specimen using CLONTECH PCR-Select cDNA Subtraction kit. |
| Authors | Liu,N.Y., Ko,S.S., Yeh,K.C., Charng,Y.Y., Lin, W.C., Cheng,M.L., Ting,H.M. and Cheng,C.P. |
| Contact Information |
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