Clone hba-31m5

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Clone
Clone name
hba-31m5
LE_HBa0031M05
C03HBa0031M05
P031M05
Clone type
BAC
Contamination
none
Estimated length
151,440
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg1199 : [coherent], 11 members:
C03HBa0031M05, C03HBa0034O02, C03HBa0070P03, C03HBa0174D19, C03HBa0190L06, C03HBa0223J13, C03HBa0239J12, C03HBa0257C07, C03HBa0277G02, C03HBa0288D21, C03HBa0322O07
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
T1086Tomato-EXPEN 2000plate 6C9
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 3
FISH map 
View on FISH map chromosome 3
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
342-04 1CompositeFluorescencePhase61.5%
342-06 1CompositeFluorescencePhase62.6%
342-07 1CompositeFluorescencePhase58.2%
342-08 1CompositeFluorescencePhase58.9%
342-09 1CompositeFlourescencePhase60.1%
342-19 1CompositeFluorescencePhase64.3%
342-20 1CompositeFluorescencePhase60.2%
342-23 1CompositeFluorescencePhase66.8%
342-24 1CompositeFluorescencePhase62.1%
342-28 1CompositeFluorescencePhase62.5%
342-34 1CompositeFluorescencePhase64.5%
342-35 1CompositeFluorescencePhase63.7%
342-44 1CompositeFluorescencePhase63.8%
342-46 1CompositeFluorescencePhase61.8%
342-48 1CompositeFluorescencePhase57.5%
342-49 1CompositeFluorescencePhase63.1%
342-50 1CompositeFluorescencePhase63.8%
Mean±sd62.1%±2.5%

Given an estimated length of 4.8µm for chromosome 3P and the mean percentage above, this BAC is approximately 3.0µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 3 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 3
HTGS phase
2 - fragmented, ordered
Sequencing Status
complete
Sequencing Organization
Uploaded by account 'jianfeng-ren'
Full Sequence
163,724 bp
C03HBa0031M05.1
AC238561.1 (GenBank) 		[View]
[Download fasta]
[Download full submission] (334.62 MB)
End Sequences
Warnings
  • sequence is not finished to HTGS3
 FTP
Chromosome 3 Sequencing repository (FTP)
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (33)
1504, 1565, 1633, 1785, 2034, 2216, 2338, 2478, 2478, 2541, 2804, 2804, 2916, 2916, 2982, 3107, 3274, 3324, 3593, 3973, 4478, 4636, 5446, 5446, 5866, 6022, 7183, 7521, 8039, 8039, 11236, 12070, 13193
HindIII in silico (41)
1086, 1095, 1102, 1126, 1229, 1497, 1514, 1573, 1639, 1775, 2013, 2216, 2352, 2478, 2508, 2556, 2801, 2812, 2839, 2914, 2932, 2995, 3115, 3280, 3309, 3351, 3623, 4010, 4261, 4451, 4564, 5134, 5180, 5554, 5751, 7142, 7214, 7432, 11143, 11893, 12899
Fragment Lengths Match Score
0.88
Annotations  
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LE_HBa0031M05 has no browsable sequence annotations
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End Sequences

e.g. crystallin
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Map
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IL Bin Mapping
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 Verification

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