Clone hba-163o4
 Clone and library
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Clone and library
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| Physical mapping
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| Fingerprint Contig Builds (FPC) |
- Tomato FPC (AGI 2005)
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ctg1175 : [coherent], 10 members:
C12HBa0041B09, C12HBa0093B17, C12HBa0121O19, C12HBa0163O04, C12HBa0172G20, C12HBa0211A04, C12HBa0236E19, C12HBa0237F11, C12HBa0297J24, C12HBa0310J15
| Marker matches - overgo what's this? |
Plausible matches
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Additional Info | ||||||||
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Conflicted matches
None
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| Marker matches - BLAST |
| Marker matches - manual |
| Physical map (from marker matches) |
| FISH map |
| FISH images |
| SC Spread ID | Images | Distance from centromere (percentage of arm length) | ||
|---|---|---|---|---|
| 664-05 1 | Composite | Fluorescence | Phase | 84.0% |
| 664-06 1 | Composite | Fluorescence | Phase | 79.3% |
| 664-07 1 | Composite | Fluorescence | Phase | 82.6% |
| 664-29 1 | Composite | Fluorescence | Phase | 81.7% |
| 664-30 1 | Composite | Fluorescence | Phase | 81.7% |
| 664-35 1 | Composite | Fluorescence | Phase | 79.1% |
| 664-37 1 | Composite | Fluorescence | Phase | 86.8% |
| 664-38 1 | Composite | Fluorescence | Phase | 83.2% |
| 664-58 1 | Composite | Fluorescence | Phase | 81.4% |
| 664-61 1 | Composite | Fluorescence | Phase | 86.0% |
| 664-62 1 | Composite | Fluorescence | Phase | 81.7% |
| 664-74 1 | Composite | Fluorescence | Phase | 84.1% |
| 664-75 1 | Composite | Fluorescence | Phase | 83.0% |
| Mean±sd | 82.7%±2.3% | |||
Given an estimated length of 6.8µm for chromosome 12P and the mean percentage above, this BAC is approximately 5.6µm±0.2µm from the chromosome centromere.
1Experiment conducted in the lab of Stephen Stack at Colorado State University.
| IL Mapping |
| Sequencing
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This clone is being sequenced by the Chromosome 12 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 12
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HTGS phase
2 - fragmented, ordered
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Sequencing Status
complete
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Sequencing Organization
not recorded
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Full Sequence
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End Sequences
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Warnings
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FTP | ||
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Chromosome Assembly
this clone is not part of a chromosome assembly
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Restriction Fragments
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| Annotations
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Browse
LE_HBa0163O04 has no browsable sequence annotations
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Download
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| User comments
| [Add comment] |
This BAC was sequenced by the chromosome 12 project and re-assigned to the chromosome 7 project after FISH indicated it was located on chromosome 7. | ||
Although an initial IL screen with the T7 end revealed that this BAC could be on chromosome 7, careful analyses performed in Italy showed that the clone is more likely located on chromosome 12. The SP6 end was bin-mapped to chromosome 12, and extended; the extension BAC also bin-mapped on chromosome 12. A further extension of that clone was identified that had already been bin-mapped to chr 12. In addition, an extension BAC was selected from the full sequence that overlaps more than 40kb, and that BAC was verified to be on chromosome 12. Thus, it is possible that a very small section of the end of this clone (T7 end) may be chimeric. The BAC has thus first been moved from chr 12 to chr 7 and in light of this evidence moved back to chr 12. (as communicated by Alessandro Vezzi, U Padua). |
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