Clone hba-177f12

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Clone
Clone name
hba-177f12
LE_HBa0177F12
C02HBa0177F12
P177F12
Clone type
BAC
Contamination
none
Estimated length
120,583
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg1810 : [coherent], 5 members:
C02HBa0051O23, C02HBa0131F11, C02HBa0177F12, C02HBa0233G16, C02HBa0265A18
Marker matches - overgo what's this? 
Plausible matches Additional Info
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
7-10 1CompositeFluorescencePhase100.0%
7-11 1CompositeFluorescencePhase100.0%
7-12 1CompositeFluorescencePhase98.5%
7-4 1CompositeFluorescencePhase100.0%
7-5 1CompositeFluorescencePhase100.0%
7-7 1CompositeFluorescencePhase100.0%
7-8 1CompositeFluorescencePhase100.0%
Mean±sd99.8%±0.6%

Given an estimated length of 18.9µm for chromosome 2Q and the mean percentage above, this BAC is approximately 18.9µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 2 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 2
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
Korea Research Institute of Bioscience and Biotechnology
Full Sequence
150,428 bp
C02HBa0177F12.3
AC215410.1 (GenBank)
[View]
[Download fasta]
[Download full submission] (223.48 MB)
End Sequences
Warnings
none
FTP
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (30)
1078, 1278, 1542, 1699, 1699, 1728, 1842, 1969, 2163, 2441, 2441, 2801, 2978, 3014, 3184, 3184, 3492, 3813, 3961, 4087, 4645, 5224, 6124, 6290, 6339, 6735, 6844, 8583, 8767, 10638
HindIII in silico (35)
1077, 1272, 1542, 1677, 1688, 1718, 1823, 1950, 2160, 2188, 2437, 2463, 2820, 2970, 3035, 3178, 3185, 3499, 3821, 3839, 3876, 3966, 4029, 4130, 4545, 4996, 5903, 6068, 6153, 6647, 6796, 8513, 8843, 10373, 10502
Fragment Lengths Match Score
0.92
Annotations  
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LE_HBa0177F12 has no browsable sequence annotations
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