Clone hba-12d17

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Clone
Clone name
hba-12d17
LE_HBa0012D17
C10HBa0012D17
P012D17
Clone type
BAC
Contamination
none
Estimated length
163,431
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg739 : [coherent], 21 members:
C10HBa0012D17, LE_HBa0023N24, LE_HBa0025F04, LE_HBa0041P01, C10HBa0116G06, LE_HBa0144K11, LE_HBa0144M21, LE_HBa0145L02, LE_HBa0145L07, LE_HBa0155L18, LE_HBa0176J20, LE_HBa0178H24, LE_HBa0182E22, LE_HBa0192C12, LE_HBa0229I22, LE_HBa0254M12, LE_HBa0292A11, LE_HBa0293J06, LE_HBa0316L18, LE_HBa0317B16, LE_HBa0322M11
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
C2_At5g06430Tomato-EXPEN 2000plate 13D12
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 10
FISH map 
View on FISH map chromosome 10
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
446-02 1CompositeFluorescencePhase90.9%
446-03 1CompositeFluorescencePhase87.2%
444-03 1CompositeFluorescencePhase85.5%
444-04 1CompositeFluorescencePhase86.2%
446-07 1CompositeFluorescencePhase88.1%
444-07 1CompositeFluorescencePhase87.0%
446-09 1CompositeFluorescencePhase89.9%
444-09 1CompositeFluorescencePhase84.4%
444-11 1CompositeFluorescencePhase86.2%
446-12 1CompositeFluorescencePhase87.0%
446-13 1CompositeFluorescencePhase87.2%
446-14 1CompositeFluorescencePhase84.4%
444-15 1CompositeFluorescencePhase86.5%
446-15a 1CompositeFluorescencePhase89.1%
446-15b 1CompositeFluoresencePhase87.1%
446-16 1CompositeFluorescencePhase90.3%
446-18 1CompositeFluorescencePhase89.1%
446-20 1CompositeFluorescencePhase86.5%
446-21 1CompositeFluorescencePhase87.4%
446-23 1CompositeFluorescencePhase86.7%
446-24 1CompositeFluorescencePhase86.4%
446-26a 1CompositeFluorescencePhase85.7%
446-26b 1CompositeFluorescencePhase88.6%
Mean±sd87.3%±1.7%

Given an estimated length of 4.8µm for chromosome 10P and the mean percentage above, this BAC is approximately 4.2µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 10 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 10
HTGS phase
3 - finished
Sequencing Status
in_progress
Sequencing Organization
Uploaded by account 'manual'
Full Sequence
151,015 bp
C10HBa0012D17.3
AC233122.7 (GenBank) 		[View]
[Download fasta]
[Download full submission] (0.10 MB)
End Sequences
Warnings
none
FTP
Chromosome 10 Sequencing repository (FTP)
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (28)
1088, 1088, 1245, 1504, 1609, 1643, 1924, 1985, 2221, 2330, 2577, 2800, 2942, 3384, 3483, 3934, 3994, 4613, 5097, 5097, 5317, 6458, 6575, 7790, 10804, 11435, 27258, 33236
HindIII in silico (30)
1086, 1086, 1127, 1251, 1495, 1505, 1597, 1655, 1929, 1985, 2231, 2355, 2575, 2796, 2931, 3422, 3515, 4030, 4090, 4590, 4916, 4939, 5126, 6447, 6492, 6669, 7712, 11105, 11382, 30433
Fragment Lengths Match Score
0.95
Annotations  
Browse
LE_HBa0012D17 has no browsable sequence annotations
Download
temporarily unavailable
User commentsNone[Add comment] 
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Estimated Length
 and  bp
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End Sequences

e.g. crystallin
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Map
Map position
 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

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