Clone hba-146i19

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Clone
Clone name
hba-146i19
LE_HBa0146I19
C12HBa0146I19
P146I19
Clone type
BAC
Contamination
none
Estimated length
99,689
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg2839 : [coherent], 3 members:
C12HBa0120O09, C12HBa0146I19, C12HBa0244C09
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
T1667Tomato-EXPEN 2000plate 4C5
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 12
FISH map 
View on FISH map chromosome 12
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
649-07 1CompositeFluorescencePhase79.0%
649-10 1CompositeFluorescencePhase76.8%
649-16 1CompositeFluorescencePhase77.1%
649-19 1CompositeFluorescencePhase77.6%
649-22 1CompositeFluorescencePhase81.4%
649-24a 1CompositeFluorescencePhase76.7%
649-24b 1CompositeFluorescencePhase77.4%
649-26 1CompositeFluorescencePhase82.3%
649-28 1CompositeFluorescencePhase77.4%
649-33 1CompositeFluorescencePhase78.7%
649-35 1CompositeFluorescencePhase79.0%
649-40 1CompositeFluorescencePhase76.2%
649-51 1CompositeFluorescencePhase82.5%
649-52 1CompositeFluorescencePhase77.4%
649-54 1CompositeFluorescencePhase81.3%
649-59 1CompositeFluorescencePhase81.3%
Mean±sd78.9%±2.2%

Given an estimated length of 6.8µm for chromosome 12P and the mean percentage above, this BAC is approximately 5.4µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 12 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 12
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
not recorded
Full Sequence
122,513 bp
C12HBa0146I19.1
AC209588.1 (GenBank) 		[View]
[Download fasta]
[Download full submission] (282.01 MB)
End Sequences
Warnings
none
FTP
Chromosome 12 Sequencing repository (FTP)
Chromosome Assembly
View on AGP map chromosome 12
  
Restriction Fragments
HindIII in vitro (23)
2005, 2307, 2542, 2561, 2740, 2891, 2891, 3091, 3158, 3436, 3460, 3610, 3890, 3890, 4049, 4049, 4559, 5854, 6375, 6375, 6520, 9037, 10399
HindIII in silico (32)
1056, 1369, 1382, 1384, 1468, 1558, 1782, 1843, 1964, 2345, 2566, 2600, 2767, 2894, 2918, 3059, 3179, 3428, 3489, 3617, 3843, 3942, 3964, 4035, 4093, 4534, 5532, 6185, 6203, 6375, 8910, 10102
Fragment Lengths Match Score
0.82
Annotations  
Browse
LE_HBa0146I19 has no browsable sequence annotations
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 and  bp
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End Sequences

e.g. crystallin
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Map
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 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

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