Clone hba-147f10

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Clone
Clone name
hba-147f10
LE_HBa0147F10
C05HBa0147F10
P147F10
Clone type
BAC
Contamination
none
Estimated length
88,881
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg3312 : [coherent], 2 members:
LE_HBa0060M21, C05HBa0147F10
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
T1790Tomato-EXPEN 2000plate 11E9
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 5
FISH map 
View on FISH map chromosome 5
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
239-13 1CompositeFluorescencePhase66.5%
239-14 1CompositeFluorescencePhase67.3%
239-15 1CompositeFluorescencePhase65.9%
239-17 1CompositeFluorescencePhase65.9%
239-20 1CompositeFluorescencePhase69.8%
239-22 1CompositeFluorescencePhase64.4%
239-23 1CompositeFluorescencePhase68.9%
239-25 1CompositeFluorescencePhase70.7%
239-27 1CompositeFluorescencePhase68.1%
239-28 1CompositeFluorescencePhase68.3%
239-29 1CompositeFluorescencePhase66.8%
239-31 1CompositeFluorescencePhase70.6%
239-36 1CompositeFluorescencePhase64.4%
239-38 1CompositeFluorescencePhase65.7%
Mean±sd67.4%±2.1%

Given an estimated length of 7.4µm for chromosome 5P and the mean percentage above, this BAC is approximately 5.0µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 5 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 5
HTGS phase
2 - fragmented, ordered
Sequencing Status
in_progress
Sequencing Organization
Indian Initiative on Tomato Genome Sequencing
Full Sequence
108,709 bp
C05HBa0147F10.1
AC232702.1 (GenBank) 		[View]
[Download fasta]
[Download full submission] (440.11 MB)
End Sequences
Warnings
  • sequence is not finished to HTGS3
 FTP
Chromosome 5 Sequencing repository (FTP)
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (27)
1491, 1601, 1638, 1723, 1791, 1974, 2053, 2099, 2518, 2748, 2791, 2834, 2933, 3305, 3305, 3524, 3629, 3739, 3739, 3812, 4109, 4109, 4288, 4627, 5782, 6292, 6427
HindIII in silico (34)
1033, 1037, 1174, 1216, 1277, 1383, 1471, 1499, 1598, 1706, 1794, 1941, 2041, 2099, 2436, 2544, 2767, 2814, 2843, 2953, 3332, 3336, 3499, 3762, 3770, 3797, 3840, 3880, 4093, 4302, 4561, 5412, 6123, 7054
Fragment Lengths Match Score
0.87
Annotations  
Browse
LE_HBa0147F10 has no browsable sequence annotations
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End Sequences

e.g. crystallin
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Map
Map position
 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

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