Clone hba-162g22

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Clone
Clone name
hba-162g22
LE_HBa0162G22
C01HBa0162G22
P162G22
Clone type
BAC
Contamination
none
Estimated length
129,375
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg3346 : [coherent], 3 members:
LE_HBa0056M19, C01HBa0162G22, LE_HBa0327C24
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
T1072Tomato-EXPEN 2000plate 3B5
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 1
FISH map 
View on FISH map chromosome 3
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
655-02 1CompositeFluorescencePhase19.9%
655-03 1CompositeFluorescencePhase15.6%
655-09 1CompositeFluorescencePhase13.7%
655-11 1CompositeFluorescencePhase20.8%
655-19 1CompositeFluorescencePhase17.3%
655-25 1CompositeFluorescencePhase17.9%
655-28 1CompositeFluorescencePhase16.0%
655-34 1CompositeFluorescencePhase13.3%
655-45 1CompositeFluorescencePhase15.2%
655-51 1CompositeFluorescencePhase13.0%
655-53 1CompositeFluorescencePhase16.9%
655-57 1CompositeFluorescencePhase14.5%
Mean±sd16.2%±2.5%

Given an estimated length of 4.8µm for chromosome 3P and the mean percentage above, this BAC is approximately 0.8µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 1 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 1
HTGS phase
3 - finished
Sequencing Status
in_progress
Sequencing Organization
Uploaded by account 'manual'
Full Sequence
148,660 bp
C01HBa0162G22.7
AC233126.17 (GenBank) 		[View]
[Download fasta]
[Download full submission] (0.09 MB)
End Sequences
Warnings
none
FTP
Chromosome 1 Sequencing repository (FTP)
Chromosome Assembly
View on AGP map chromosome 1
  
Restriction Fragments
HindIII in vitro (28)
1955, 1955, 2200, 2312, 2312, 2439, 2471, 2828, 3020, 3092, 3092, 3123, 3326, 3810, 4147, 4627, 4784, 5176, 5288, 5451, 5451, 5797, 5843, 6430, 7471, 9747, 10235, 10993
HindIII in silico (36)
1026, 1207, 1220, 1408, 1425, 1444, 1835, 1915, 1915, 2211, 2341, 2349, 2444, 2502, 2846, 3012, 3052, 3093, 3093, 3105, 3167, 3337, 3897, 4183, 4543, 4661, 4941, 5067, 5171, 5484, 5529, 6307, 7374, 9553, 10271, 10807
Fragment Lengths Match Score
0.85
Annotations  
Browse
LE_HBa0162G22 has no browsable sequence annotations
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temporarily unavailable
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Estimated Length
 and  bp
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Annotations
End Sequences

e.g. crystallin
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Map
Map position
 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

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