Clone hba-262o22

Clone and library  
Clone
Clone name
hba-262o22
LE_HBa0262O22
C01HBa0262O22
P262O22
Clone type
BAC
Contamination
none
Estimated length
44,800
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
not present in Tomato FPC (AGI 2005)
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
T1650Tomato-EXPEN 2000plate 3F8
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 1
FISH map 
View on FISH map chromosome 1
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
195-17 1CompositeFluorescencePhase80.0%
195-18 1CompositeFluorescencePhase78.8%
195-19 1CompositeFluorescencePhase79.7%
195-20 1CompositeFluorescencePhase78.7%
195-22 1CompositeFluorescencePhase79.7%
195-24 1CompositeFluorescencePhase82.0%
195-26 1CompositeFluorescencePhase79.3%
Mean±sd79.7%±1.1%

Given an estimated length of 6.7µm for chromosome 1P and the mean percentage above, this BAC is approximately 5.3µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 1 Sequencing Project. (View projects)
Log in as a curator or sequencer to edit this clone's registry information.
Sequencing Project
Chromosome 1
HTGS phase
1 - fragmented, unordered
Sequencing Status
complete
Sequencing Organization
Uploaded by account 'manual'
Full Sequence
81,476 bp
C01HBa0262O22.1
AC225025.1 (GenBank) 		[View]
[Download fasta]
[Download full submission] (97.39 MB)
End Sequences
None
Warnings
  • sequence is not finished to HTGS3
  • poor correspondence with in vitro restriction fragments
    (match score below 0.7)
  • sequence length 81,476 is 81.9% different from estimated length 44,800
 FTP
Chromosome 1 Sequencing repository (FTP)
Chromosome Assembly
View on AGP map chromosome 1
  
Restriction Fragments
HindIII in vitro (8)
3135, 3197, 3443, 5322, 5536, 5926, 6223, 12018
HindIII in silico (20)
1403, 1536, 1545, 1791, 2257, 2312, 2381, 2398, 2486, 2778, 3260, 3297, 3621, 3880, 4522, 5087, 5262, 5959, 7311, 14371
Fragment Lengths Match Score
0.59
Annotations  
Browse
LE_HBa0262O22 has no browsable sequence annotations
Download
 
User commentsNone[Add comment] 
Search again  
Genomic Clone Search Select a genomic clone at random

full names, partial names, or lists of both

 More Criteria

Seq. Center Reported Status
Estimated Length
 and  bp
GenBank Accession (version insensitive)
e.g. AP009318.1 or AP009318
Sequencing on Chromosome
Show Only Clones With







Annotations
End Sequences

e.g. crystallin
Overgo match to marker what's this?
Map
Map position
 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

Related Links: