Clone hba-257n18

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Clone
Clone name
hba-257n18
LE_HBa0257N18
C03HBa0257N18
P257N18
Clone type
BAC
Contamination
none
Estimated length
96,651
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg4324 : [coherent], 2 members:
LE_HBa0106H01, C03HBa0257N18
Marker matches - overgo what's this? 
Plausible matches Additional Info
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
357-05 1CompositeFluorescencePhase76.0%
357-09 1CompositeFluorescencePhase71.3%
357-10 1CompositeFluorescencePhase74.4%
357-11 1CompositeFluorescencePhase73.8%
357-12 1CompositeFluorescencePhase70.8%
357-13a 1CompositeFluorescencePhase72.9%
357-13b 1CompositeFluorescencePhase72.3%
357-13c 1CompositeFluorescencePhase72.6%
357-14 1CompositeFluorescencePhase71.3%
357-15 1CompositeFluorescencePhase71.8%
357-16 1CompositeFluorescencePhase73.6%
357-17 1CompositeFluorescencePhase71.2%
357-18 1CompositeFluorescencePhase69.1%
357-19 1CompositeFlourescencePhase73.5%
357-21 1CompositeFluorescencePhase70.9%
357-23 1CompositeFluorescencePhase69.0%
357-26 1CompositeFluorescencePhase73.3%
357-28 1CompositeFluorescencePhase74.5%
357-30 1CompositeFluorescencePhase73.5%
357-31 1CompositeFluorescencePhase72.9%
357-33 1CompositeFluorescencePhase74.8%
Mean±sd72.5%±1.8%

Given an estimated length of 4.8µm for chromosome 3P and the mean percentage above, this BAC is approximately 3.5µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL Mapping 
Assigned to project
China
IL-mapped to chromosome
3
IL-mapped to IL segment
-
IL mapping notes
-
Sequencing  
This clone is being sequenced by the Chromosome 3 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 3
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
Uploaded by account 'jianfeng-ren'
Full Sequence
142,622 bp
C03HBa0257N18.1
AC238467.1 (GenBank)
[View]
[Download fasta]
[Download full submission] (261.63 MB)
End Sequences
None
Warnings
  • poor correspondence with in vitro restriction fragments
    (match score below 0.7)
  • sequence length 142,622 is 47.6% different from estimated length 96,651
FTP
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (10)
4473, 5043, 5043, 5912, 5938, 6390, 8651, 12597, 20434, 22170
HindIII in silico (28)
1119, 1383, 1490, 1499, 1509, 1634, 1665, 1740, 1743, 2062, 2112, 2120, 2178, 2329, 3999, 4197, 4367, 4594, 4791, 4874, 5597, 5665, 5768, 6243, 8517, 12189, 19578, 21697
Fragment Lengths Match Score
0.51
Annotations  
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LE_HBa0257N18 has no browsable sequence annotations
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