Clone Read LE_HBa0167C20_T7_12807

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0167C20_T7_12807
Primer
T7
ID from Seq. Facility
LE_HBa_167_C20-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
731 trimmed for vector and quality, 800 raw
Genbank Accession
DU031484
Sequence
>LE_HBa0167C20_T7_12807 (raw untrimmed)
CTCCGGTCCCGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTACGTTTTAGTTCAACTTTGACGCATTAGCTATGGTGGGCTCTGGGCAATG
CTCACATACCACCGGATGTTCACTTCCATTGACTGTTATATTTAGCTGGGGATGCTGAAGGGTAAGTACGTGATAAGATCAAAACGTTAGTGACTCTTAA
GTGTGCTTGCTTTCTATGATATAACTTGCCTTTCACATCATAGGAATGCCCAGAGTATCGGTGGGCTCAATCTATATAGGAGCTTTTAATGACTGCAGTG
CTGTTTCCTTTGGCCAGAAAGTTCTAGTATTCCTTTCTATTTAGCTGAATATCCTAATGTTCTCTTGCAGCTTGCTTGGACTTGCTATAACTTTTACCAG
TCAACACCTACAAAATTGGCAGGAGAGAACTATTTTTTTAATGACGGCCAAGTCAGTGTTTTCAGTTTTAGTTCAGGATGATATTTGTTTTTGTTGTTTG
CTAATGGTGATAACTGATAAGTTATTACTTCAATTGTTCATCCTGTTTAATAATCTTCAGGATATGAGTGTGGGCACATCATGGAACATATTAAGGCCAG
AGACAGTTGAGTCACTGTTTTACCTCTGGCGTTTAACAGGAAACAAGACATACCAAGAGTGGGGTTGGAACATATTTCAAGCATTTGAAAAGAACTCAAG
GGTAGAGTCTGGATATGTTGGACTTAAGATGTAAGCTCCAAATCAATTCTTTTAGGTGCATTTTTTTATTCTGTTTCTGGCATTAAGAAACTGCTGCTAT
>LE_HBa0167C20_T7_12807 (trimmed for quality and cloning vector)
AGCTTTACGTTTTAGTTCAACTTTGACGCATTAGCTATGGTGGGCTCTGGGCAATGCTCACATACCACCGGATGTTCACTTCCATTGACTGTTATATTTA
GCTGGGGATGCTGAAGGGTAAGTACGTGATAAGATCAAAACGTTAGTGACTCTTAAGTGTGCTTGCTTTCTATGATATAACTTGCCTTTCACATCATAGG
AATGCCCAGAGTATCGGTGGGCTCAATCTATATAGGAGCTTTTAATGACTGCAGTGCTGTTTCCTTTGGCCAGAAAGTTCTAGTATTCCTTTCTATTTAG
CTGAATATCCTAATGTTCTCTTGCAGCTTGCTTGGACTTGCTATAACTTTTACCAGTCAACACCTACAAAATTGGCAGGAGAGAACTATTTTTTTAATGA
CGGCCAAGTCAGTGTTTTCAGTTTTAGTTCAGGATGATATTTGTTTTTGTTGTTTGCTAATGGTGATAACTGATAAGTTATTACTTCAATTGTTCATCCT
GTTTAATAATCTTCAGGATATGAGTGTGGGCACATCATGGAACATATTAAGGCCAGAGACAGTTGAGTCACTGTTTTACCTCTGGCGTTTAACAGGAAAC
AAGACATACCAAGAGTGGGGTTGGAACATATTTCAAGCATTTGAAAAGAACTCAAGGGTAGAGTCTGGATATGTTGGACTTAAGATGTAAGCTCCAAATC
AATTCTTTTAGGTGCATTTTTTTATTCTGTT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.964872
Expected error %
0.598754
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g51590.1 Score 123 E Value 2e-40 Identity 100.00% Align. Length 360bp (49.25%) Frame 1
68414.m05808 mannosyl-oligosaccharide 1,2-alpha-mannosidase, putative similar to mannosyl-oligosaccharide 1,2-alpha-mannosidase [Glycine max][GI:6552504]
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g21160.1 Score 121 E Value 3e-40 Identity 96.40% Align. Length 360bp (49.25%) Frame 1
68416.m02673 mannosyl-oligosaccharide 1,2-alpha-mannosidase, putative similar to mannosyl-oligosaccharide 1,2-alpha-mannosidase [Glycine max][GI:6552504]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAM91245.1| Score 123 E Value 9e-39 Identity 100.00% Align. Length 360bp (49.25%) Frame 1
mannosyl-oligosaccharide 1,2-alpha-mannosidase, putative [Arabidopsis thaliana] gb|AAL91242.1| mannosyl-oligosaccharide 1,2-alpha-mannosidase, putative [Arabidopsis thaliana] ref|NP_175570.1| mannosyl-oligosaccharide 1,2-alpha-mannosidase, putative [Arabidopsis thaliana] gb|AAG50876.1| mannosyl-oligosaccharide alpha-1,2-mannosidase, putative [Arabidopsis thaliana] gb|AAG52623.1| mannosyl-oligosaccharide 1,2-alpha-mannosidase, putative; 8615-12432 [Arabidopsis thaliana] pir||E96554 hypothetical protein F19C24.18 [imported] - Arabidopsis thaliana
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAF16414.1| Score 122 E Value 6e-39 Identity 98.20% Align. Length 360bp (49.25%) Frame 1
mannosyl-oligosaccharide 1,2-alpha-mannosidase [Glycine max]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0167C20
Clone
Clone name
hba-167c20
LE_HBa0167C20
C03HBa0167C20
P167C20
Clone type
BAC
Contamination
none
Estimated length
38,104
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found