Clone Read LE_HBa0023E12_SP6_134219

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0023E12_SP6_134219
Primer
SP6
ID from Seq. Facility
LE_HBa_023_E12-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
640 trimmed for vector and quality, 897 raw
Sequence
>LE_HBa0023E12_SP6_134219 (raw untrimmed)
AAAAAATGGTTTAGGCGCTCACAANCAATACACTCCTTCTCAAGGAATTAAAATNTCGTAGCAAAATTATAAAATGAATACAATAAAGACAAGTGTGCAA
GAAATCAACGAAAATGATATCAATTTAAGAAAATACATATAATTTCTCAAATTCCTATTTATATGTCTTTATTTTAGATTTCAACTTATTAAGAAATTAG
ATAAGTTTATTTATAGTAATCTCTTGAAGTAAAGCTTTCAGTAAATGAATATAAATTTAATTTATTTTGATTGATTAAATCGATCAATGAACATAAATTA
AGTATTTGATTTTCTTATGTTGGTCAAGTTCATATTTTTAAGGCTAATAACTCTCAAGGGTAAAAGAGAAAATAGCGTTATTTATATACTCCCTTCTTTT
CAAAAAGGATGACCTAGTTATGTAATTATTGGAATAATTGTTGGAGATTCAAATGATGGATATGCAAGCGACTTGGAATGGAGTTTAATAAAAGAAAGAA
GACTTTTTAATCTTGTGGTCTAAATTAAAGTTATGTTAAATGTACCAAAATGTTCTTTAATCCTACGTGGAAAGTTAAAATTAAAGTGTTACCAAAAACA
GAAAGGGATCATTCTTTTTGAAACAGACTAAAAAAAATAGGGTCGTTCTTTTTTAAACGGGGGGAGTATTAATTTTATGAAATGACAAGTACTCCCTCTG
TTCCTAATTACTTGTCTACTTTTGAATTGACATATAAATTAAATATTAAGCATATTGAGTTTACCCTTTTTACCTCTATTAATTTATGAGGTGGATAAAA
TAAAAAAATTTAAGATGGTTCAAGACCCTTTTTCAAAGTAATTTAATTGAAGGAAATTAAAAAAAAAAAAAAGTTTTGTCCCTTTATTTAAATTTGT
>LE_HBa0023E12_SP6_134219 (trimmed for quality and cloning vector)
GTAGCAAAATTATAAAATGAATACAATAAAGACAAGTGTGCAAGAAATCAACGAAAATGATATCAATTTAAGAAAATACATATAATTTCTCAAATTCCTA
TTTATATGTCTTTATTTTAGATTTCAACTTATTAAGAAATTAGATAAGTTTATTTATAGTAATCTCTTGAAGTAAAGCTTTCAGTAAATGAATATAAATT
TAATTTATTTTGATTGATTAAATCGATCAATGAACATAAATTAAGTATTTGATTTTCTTATGTTGGTCAAGTTCATATTTTTAAGGCTAATAACTCTCAA
GGGTAAAAGAGAAAATAGCGTTATTTATATACTCCCTTCTTTTCAAAAAGGATGACCTAGTTATGTAATTATTGGAATAATTGTTGGAGATTCAAATGAT
GGATATGCAAGCGACTTGGAATGGAGTTTAATAAAAGAAAGAAGACTTTTTAATCTTGTGGTCTAAATTAAAGTTATGTTAAATGTACCAAAATGTTCTT
TAATCCTACGTGGAAAGTTAAAATTAAAGTGTTACCAAAAACAGAAAGGGATCATTCTTTTTGAAACAGACTAAAAAAAATAGGGTCGTTCTTTTTTAAA
CGGGGGGAGTATTAATTTTATGAAATGACAAGTACTCCCT

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.903373
Expected error %
0.292
Sequence Annotations 
Automatic Annotations
Nicotiana tabacum Chloroplast (screening/organelle/nicotiana_tabacum_chloroplast)NCBI BLAST
gi|11465934|ref|NC_001879.1| Score 94 E Value 6e-21 Identity 98.00% Align. Length 50bp (7.81%) Frame 3
Nicotiana tabacum plastid, complete genome
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0023E12
Clone
Clone name
hba-23e12
LE_HBa0023E12
P023E12
Clone type
BAC
Contamination
none
Estimated length
107,022
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found