Clone Read LE_HBa0026F14_SP6_136575

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0026F14_SP6_136575
Primer
SP6
ID from Seq. Facility
LE_HBa_026_F14-SP6
Chromatogram
Sequence 
Version
1 (current)
Length (bp)
687 trimmed for vector and quality, 958 raw
Sequence
>LE_HBa0026F14_SP6_136575 (raw untrimmed)
AAAAATGGGGTTCCCCCCTCTATGTACAANCNNACCCANGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCGTTATCTTTTAAAAGATGTGC
CGCCCAAGCGAAACTCCCCACCTGACAATGTCTTCTGCACGGATCGGCCCGCGAAGTGAGCCTTTGGTCCAAAAAGAGGGGCAGTGCCCCACTTCCGATT
CACGGAATAAGTAAAATAACGTTTAAAGTAGTGGTATTTCACTTTCGCCTTTCGACTCCCACTTATATTACACCTCTCAAGTCATTTGATAAAGTCGGAC
TAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCTTCTAAGCCCGTTCGCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATC
TCGTTAATCCATTCATGCACGTCACTAATTAGATTACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTACTCCTGCCGTTTACCCGCGCTTGGTTGAA
TTTCTTCACTTTGACATTCAGAGCACTGTGCAGAAATCACATTGCGTAAACATCCGTTGCGACCATCGCAATGCTTTGTTTAAATTAAACAGTCGGATTC
CCCTTGTCCGTACAAGTTCTGAGTTGTTTGTTCGACACCCGAAGAAGGCCCCCGAAGGAACCGTTCCTAGTCCTTCCCCCGTCCGGCACGCGGCAACACG
CTCTCGCCGTGGGAGCTGCTCGAGCAGTCCACCGACAGCCGAAGGGTTTGGGACTTGGATCCCCGTGCACAGCCCTCCCAGCCAATCCTTTTCCCAAAGG
GACGGATCATATTTGCCAACTTCCCTTGCCTCATTGTTCATCCAACAGAAGTTGTTTCCTTGGAAACTTGATGCGGTTTTAATACCATCCGGCATGGACG
GCTTCGGTCCTCCGAATTTTTAGGCCGCCTGGAAACACGGAAACCCACCAGTTCGGGC
>LE_HBa0026F14_SP6_136575 (trimmed for quality and cloning vector)
GATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCGTTATCTTTTAAAAGATGTGCCGCCCAAGCGAAACTCCCCACCTGACAATGTCTTCTGCACG
GATCGGCCCGCGAAGTGAGCCTTTGGTCCAAAAAGAGGGGCAGTGCCCCACTTCCGATTCACGGAATAAGTAAAATAACGTTTAAAGTAGTGGTATTTCA
CTTTCGCCTTTCGACTCCCACTTATATTACACCTCTCAAGTCATTTGATAAAGTCGGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCT
TCTAAGCCCGTTCGCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCATGCACGTCACTAATTAGATTACGAGG
CATTTGGCTACCTTAAGAGAGTCATAGTTACTCCTGCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTCAGAGCACTGTGCAGAAATCACA
TTGCGTAAACATCCGTTGCGACCATCGCAATGCTTTGTTTAAATTAAACAGTCGGATTCCCCTTGTCCGTACAAGTTCTGAGTTGTTTGTTCGACACCCG
AAGAAGGCCCCCGAAGGAACCGTTCCTAGTCCTTCCCCCGTCCGGCACGCGGCAACACGCTCTCGCCGTGGGAGCTGCTCGAGCAGT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.988537
Expected error %
0.1272
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
pir||T02995 Score 251 E Value 1e-65 Identity 25.20% Align. Length 575bp (83.70%) Frame 2
unspecific monooxygenase (EC 1.14.14.1) - common tobacco dbj|BAA10929.1| cytochrome P450 like_TBP [Nicotiana tabacum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
pir||T02955 Score 214 E Value 2e-54 Identity 22.60% Align. Length 584bp (85.01%) Frame 2
probable cytochrome P450 monooxygenase - maize (fragment)
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0026F14
Clone
Clone name
hba-26f14
LE_HBa0026F14
C02HBa0026F14
P026F14
Clone type
BAC
Contamination
none
Estimated length
155,467
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found