Clone Read LE_HBa0028K10_T7_138344

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0028K10_T7_138344
Primer
T7
ID from Seq. Facility
LE_HBa_028_K10-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
596 trimmed for vector and quality, 992 raw
Genbank Accession
DU047838
Sequence
>LE_HBa0028K10_T7_138344 (raw untrimmed)
GCGTATATGGTTCTGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTTTACCTTTTCATGGAATTCCATGCCATTAAAATTTATAATTTCGTC
GTTACATTCATTTTATTTTGAAGTTCATATAATCTAGGTTTACTTGTTTAGTGCTTGCTTTGATTTATCAGGTTGTTTTGTCGTTAGAAAATGAAGACTC
ATTCTGATATTAGTAATTTGACATTTGACACACATATCTCCAGACTTTATCAGTCCATGTTTCTATAACGGCATAAGCAGAAACAAGGCTGGTGCATTAC
AATCATTGTTAAGGAATGAATACAGACGTTGAGCTAAATTTTCACCTCGTAACTGCACAGATCTCCTGGATCCGAGTTTATTGATTTTTGGATTTGAGAG
ACTTACATTGTAATATAGGGAGGTGGAGCCATTTGAATCACCAAGTGACTTACAATGATGATCAGCAAAGAGCTTTTATTGACTTACGTTTATCTCCTTG
TCTATATCGTGCTTTCATCAGGAGTTATTTTGTATAACAAGGTATGTGTCTTTTTCCAAGTATTTCATGATTGCCTTCTACTTCATTGGATTCATGGATT
GTGTTCTTTGTCAGTTAGATATGTAAATGTCTATAGCTTAAATGCATTTAAGCAACCTCAGAAAATGAAGAAGGAAATAAAATAAAATAAAGGGAAGTGA
AAACCACCATTATATTAAAATTGTGGTTTAAGCGATTTCTGAAAAGAACCAAAGTGACAGGCTGCTACCATTTATTCCATGTGCCTCCAAGGGAACTTAG
AGGGGCCAGGGTCACTTACTTTCTTGGAACCCAAAAGGAGGATAGTAAACATGCATGATTCACTATTTGCTCTTGGCCAAAACTAACTGTCTCACCTTCC
TAACGCAAGGGGAAAGAACAAATTTCTTTTTTTCCCGTCTACTACAAAACTCCTTAAGGGAAATTAATCTTTTGGTGGAGGACCTAGTTTTT
>LE_HBa0028K10_T7_138344 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTTTTACCTTTTCATGGAATTCCATGCCATTAAAATTTATAATTTCGTCGTTACATTCATTTTATTTTGAA
GTTCATATAATCTAGGTTTACTTGTTTAGTGCTTGCTTTGATTTATCAGGTTGTTTTGTCGTTAGAAAATGAAGACTCATTCTGATATTAGTAATTTGAC
ATTTGACACACATATCTCCAGACTTTATCAGTCCATGTTTCTATAACGGCATAAGCAGAAACAAGGCTGGTGCATTACAATCATTGTTAAGGAATGAATA
CAGACGTTGAGCTAAATTTTCACCTCGTAACTGCACAGATCTCCTGGATCCGAGTTTATTGATTTTTGGATTTGAGAGACTTACATTGTAATATAGGGAG
GTGGAGCCATTTGAATCACCAAGTGACTTACAATGATGATCAGCAAAGAGCTTTTATTGACTTACGTTTATCTCCTTGTCTATATCGTGCTTTCATCAGG
AGTTATTTTGTATAACAAGGTATGTGTCTTTTTCCAAGTATTTCATGATTGCCTTCTACTTCATTGGATTCATGGATTGTGTTCTTTGTCAGTTAG

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.949883
Expected error %
0.1423
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U02430.1:1751-1832 Score 66 E Value 4e-12 Identity 100.00% Align. Length 32bp (5.37%) Frame 2
Cloning vector pMAMneoBlue
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U02437.1:4662-4674-49 Score 66 E Value 4e-12 Identity 100.00% Align. Length 32bp (5.37%) Frame 2
Cloning vector pT3/T7-LUC
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0028K10
Clone
Clone name
hba-28k10
LE_HBa0028K10
C02HBa0028K10
P028K10
Clone type
BAC
Contamination
none
Estimated length
105,714
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found