Clone Read LE_HBa0179P22_T7_204274

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0179P22_T7_204274
Primer
T7
ID from Seq. Facility
LE_HBa_179_P22-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
332 trimmed for vector and quality, 877 raw
Sequence
>LE_HBa0179P22_T7_204274 (raw untrimmed)
TCGGATCCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCAACTAAACATACATGTGTTGATCTTTTGTTGTTTCATTAACAGTGCAATTCA
ACATGTACATGGCTTTTCACTTGTTGTTTCATTAACAGGGCAATTCAACATGTTACAAAGTCCAGCGTGGATGGCTGTATGACCCTAGTGTGTTATGAAC
TGTTTCTTCGAGAAGGATGATACCACCACCTATCAGGCCCGACGATTGGTCCACAGAGTTGTATCCCTGTTCATCTGGTCTAAAAATAATATCAAATGCT
TGTTTGGTTAATATCTGTAGCTCTGTTTCTTCTATTTATATCTTATATATGCTCAATTGTATTTAATTTGAATTCAACTTAAATTTCAATATGGCTATGT
CGTATCTCATTTACACTTTTAATTTTTTCTTTATTTTCATATTATATTTATCTCTAATTCTTATAAATTTCTTGTATCCTTGATATTAATACTTCCTTTC
ATTACTAGCTATCATACTTATTTATTTCAAATATTTCCATCTCTGTTCGTTTTCTTACGTATTATTATCACCTATCTGTATCATACGTCTATTGTAACTA
TATTATATTAATTTCCTTCTATCGCATTTTCTTTTTCGATATAGATTCTGTTGTTTTGTGTGATTTCTTCTTTATTCTCTCTTGTTTGCTTAGTGTAGTT
TCCTCTACCTTCTTTTTCTTTTTGCTCTTTTGTTAATTCGATTCACTTATTTTTCCTGCTCTTAATTTTATTATTATCTTGCATACCACTTTATTTTTTA
TCTCAATAGCTCCTTTCTCATTGCTACTCATATGTCACGTTTCATACTATTGTCTGCTCTCCTCTTTCTCTTTTTTG
>LE_HBa0179P22_T7_204274 (trimmed for quality and cloning vector)
AGCTTCAACTAAACATACATGTGTTGATCTTTTGTTGTTTCATTAACAGTGCAATTCAACATGTACATGGCTTTTCACTTGTTGTTTCATTAACAGGGCA
ATTCAACATGTTACAAAGTCCAGCGTGGATGGCTGTATGACCCTAGTGTGTTATGAACTGTTTCTTCGAGAAGGATGATACCACCACCTATCAGGCCCGA
CGATTGGTCCACAGAGTTGTATCCCTGTTCATCTGGTCTAAAAATAATATCAAATGCTTGTTTGGTTAATATCTGTAGCTCTGTTTCTTCTATTTATATC
TTATATATGCTCAATTGTATTTAATTTGAATT

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.948432
Expected error %
1.69529
Sequence Annotations 
Automatic Annotations
Arabidopsis thaliana Mitochondria (screening/organelle/ATH1_mitochondria)NCBI BLAST
mitochondria Score 62 E Value 3e-11 Identity 90.20% Align. Length 50bp (15.06%) Frame 3
No sequence description found.
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0179P22
Clone
Clone name
hba-179p22
LE_HBa0179P22
P179P22
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found