Genomic Clone End Sequence - Sol Genomics Network

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Clone Read LE_HBa0042M21_T7_63209

Sequence Read

Read Class

BAC ends

Read ID

LE_HBa0042M21_T7_63209

Primer

ID from Seq. Facility

LE_HBa_042_M21-T7

Chromatogram

[download]

Sequence

Version

1 (current)

Length (bp)

578 trimmed for vector and quality, 626 raw

Sequence

Show Raw Sequence

>LE_HBa0042M21_T7_63209 (trimmed for quality and cloning vector)
AGCTTCAACTTTTGGCTCCGCCTTCCTTTATGTGTATTCTTTTTTGTGCATTGTTCAAAATGTGGGATTAAATTTTCAGTTATTTTCACCTGTAGGCTGG
ACCAGGTACAATTGCAGAAGCACTAATCAGAGGGCTGCCAATTATTCTCAACGACTACATTCCCGGACAAGTTAGTGAAATCAAAACATTGATTATTCGA
CTGCTAGCTATCGTAGTATAGCTGCTGTTATGACCGTGTTTACTGATTTACATTTCCTTTATGTATTTCTCAGGAAAAGGGAAACGTTCCATACGTTGTG
GACAATGGAGCTGGTGTTTTCACACGAAGCCCCAAGGAAACAGCCCGGATTGTTGCAGAGTGGTTTAGCACCAAAGAGGATGAGCGCAAAACTATATCAG
AGAATGCACTTAAACTTGCACAACCCGATGCAGTTTTTGACATCGTGAAGGACATCCACGAGCTTGCATGCCAGAGAGGTCCTCTCGCCAACATTCCTTA
CGAGTTCACGTCTTCATTTTCAAGCTTAATTTAAGCCAGATACATAGATTCTGGTTGTTTTTTTTTGTCTTTTTTACT

Warnings:

Not screened for chimera
Not screened for repetitive elements

Quality Report
Processed by SGN	Vector signature 5' read, flanking vector found	Quality trim threshold 12.5	Sequence entropy 0.963498	Expected error % 0.410756

Sequence Annotations

Automatic Annotations

Arabidopsis proteins (TAIR10) (ath/ATH_pep)

NCBI BLAST

At5g20410.1

Score 137 E Value 1e-41 Identity 77.00% Align. Length 435bp (75.26%) Frame 1

68418.m02427 1,2-diacylglycerol 3-beta-galactosyltransferase, putative / monogalactosyldiacylglycerol synthase, putative / MGDG synthase, putative identical to monogalactosyldiacylglycerol synthase [gi:3367638] from Arabidopsis thaliana, similar to MGDG synthase type A [gi:9884651] from Glycine max

Arabidopsis proteins (TAIR10) (ath/ATH_pep)

NCBI BLAST

At2g11810.1

Score 115 E Value 3e-35 Identity 78.40% Align. Length 396bp (68.51%) Frame 1

68415.m01269 1,2-diacylglycerol 3-beta-galactosyltransferase, putative / monogalactosyldiacylglycerol synthase, putative / MGDG synthase, putative identical to monogalactosyldiacylglycerol synthase type C [gi:9927295] from Arabidopsis thaliana, similar to MGDG synthase type A [gi:9884651] from Glycine max

NCBI NR non-redundant protein sequence database (genbank/nr)

NCBI BLAST

emb|CAA04005.1|

Score 137 E Value 4e-40 Identity 77.00% Align. Length 435bp (75.26%) Frame 1

monogalactosyldiacylglycerol synthase [Arabidopsis thaliana] ref|NP_568394.2| 1,2-diacylglycerol 3-beta-galactosyltransferase, putative / monogalactosyldiacylglycerol synthase, putative / MGDG synthase, putative [Arabidopsis thaliana] pir||T52269 1,2-diacylglycerol 3-beta-galactosyltransferase (EC 2.4.1.46) [imported] - Arabidopsis thaliana

NCBI NR non-redundant protein sequence database (genbank/nr)

NCBI BLAST

gb|AAL77739.1|

Score 137 E Value 4e-40 Identity 77.00% Align. Length 435bp (75.26%) Frame 1

AT5g20410/F5O24_300 [Arabidopsis thaliana] gb|AAK50066.1| AT5g20410/F5O24_300 [Arabidopsis thaliana]

Manual Annotations

No manual annotations found.

BAC

Clone LE_HBa0042M21

Clone
Clone name hba-42m21 LE_HBa0042M21 P042M21
Clone type BAC
Contamination none
Estimated length 89,953

Library
Name Tomato HindIII BAC Library	Cloning host E. coli
Organism Solanum lycopersicum (formerly Lycopersicon esculentum)	Cloning vector pBeloBAC11
Accession Heinz 1706	Restriction enzyme HindIII

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