Clone Read LE_HBa0138D01_T7_70417

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0138D01_T7_70417
Primer
T7
ID from Seq. Facility
LE_HBa_138_D01-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
713 trimmed for vector and quality, 757 raw
Sequence
>LE_HBa0138D01_T7_70417 (raw untrimmed)
GCTCGGTACCGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCCCCAATTGGATGATTATTTGGATAAAAATTGATGTTGAACTTTTCTTTTT
CATCATCTCAATTTGCAGGTGCTTCTCTTTTCTGCAACTTTTGGGGAAAATGTCAAAGCATTTGTGACAAAAATTGTTCAAGATCTTTTCGTGCAAGATT
ACAACCAAATGTTTGTGAAGAAAGAAGAACTTTCGCTGGATTCAGTGAAGCAGTATAAAGTACAGTGTCCAGATGAACTTTCAAAAATTATGGTGATTAA
AGACAGAATACTTGAACTAGGACAGAAGGTGGGGCAAACAATTATATTCGTTCGTACCAGAAACAGTGCAAGTATGTTGCATAAGTCATTGGTTGACTAT
GGCTATGAAGTGACAACAATTCAAGGTGCCCTTAGGCAAGAAGACAGAGACAAAATAATCAAGGAGTTCAAAGATGGATTGACACAAATTCTTATATCAA
CTGATCTTCTTGCTCGGGGATTTGACCAATCACAGGTAACTTTAGATTTGAGGCTTTCCAGTTGCCAACCTTATATATGCTATACAAAGTTGTTTCTTCT
TCTGAATGTGAAGCTTGTTGTGTAGCTATAATATTTAATATCTTTTCTTTCTTGATTTCTGTCTTGGTGGCTAATATTGGGAAAATCTTAAAAGATATGA
TACTGTTTGCAGGTTAATTTGGTGGTTAATTATGACCTCCCTGTGAGACATGAGAGT
>LE_HBa0138D01_T7_70417 (trimmed for quality and cloning vector)
AGCTTCCCCAATTGGATGATTATTTGGATAAAAATTGATGTTGAACTTTTCTTTTTCATCATCTCAATTTGCAGGTGCTTCTCTTTTCTGCAACTTTTGG
GGAAAATGTCAAAGCATTTGTGACAAAAATTGTTCAAGATCTTTTCGTGCAAGATTACAACCAAATGTTTGTGAAGAAAGAAGAACTTTCGCTGGATTCA
GTGAAGCAGTATAAAGTACAGTGTCCAGATGAACTTTCAAAAATTATGGTGATTAAAGACAGAATACTTGAACTAGGACAGAAGGTGGGGCAAACAATTA
TATTCGTTCGTACCAGAAACAGTGCAAGTATGTTGCATAAGTCATTGGTTGACTATGGCTATGAAGTGACAACAATTCAAGGTGCCCTTAGGCAAGAAGA
CAGAGACAAAATAATCAAGGAGTTCAAAGATGGATTGACACAAATTCTTATATCAACTGATCTTCTTGCTCGGGGATTTGACCAATCACAGGTAACTTTA
GATTTGAGGCTTTCCAGTTGCCAACCTTATATATGCTATACAAAGTTGTTTCTTCTTCTGAATGTGAAGCTTGTTGTGTAGCTATAATATTTAATATCTT
TTCTTTCTTGATTTCTGTCTTGGTGGCTAATATTGGGAAAATCTTAAAAGATATGATACTGTTTGCAGGTTAATTTGGTGGTTAATTATGACCTCCCTGT
GAGACATGAGAGT

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.951248
Expected error %
0.0848155
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g53110.1 Score 174 E Value 6e-44 Identity 20.50% Align. Length 434bp (60.87%) Frame 1
68416.m05853 DEAD/DEAH box helicase, putative RNA helicase, Mus musculus, PIR:I49731
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g13920.1 Score 77 E Value 7e-15 Identity 11.30% Align. Length 431bp (60.45%) Frame 1
68416.m01758 eukaryotic translation initiation factor 4A-1 / eIF-4A-1 eIF-4A-1 gi:15293046, gi:15450485; contains Pfam profile PF00270: DEAD/DEAH box helicase; contains Pfam profile PF00271: Helicase conserved C-terminal domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAP68306.1| Score 174 E Value 3e-42 Identity 20.50% Align. Length 434bp (60.87%) Frame 1
At3g53110 [Arabidopsis thaliana] emb|CAB64214.1| RNA helicase-like protein [Arabidopsis thaliana] gb|AAO00804.1| RNA helicase -like protein [Arabidopsis thaliana] ref|NP_190879.1| DEAD/DEAH box helicase, putative [Arabidopsis thaliana] pir||T46157 RNA helicase-like protein - Arabidopsis thaliana
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAL09787.1| Score 174 E Value 3e-42 Identity 20.50% Align. Length 434bp (60.87%) Frame 1
AT3g53110/T4D2_40 [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0138D01
Clone
Clone name
hba-138d1
LE_HBa0138D01
P138D01
Clone type
BAC
Contamination
none
Estimated length
92,203
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found