Clone Read LE_HBa0145I04_SP6_71430

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0145I04_SP6_71430
Primer
SP6
ID from Seq. Facility
LE_HBa_145_I04-SP6
Chromatogram
Sequence 
Version
1 (current)
Length (bp)
711 trimmed for vector and quality, 828 raw
Genbank Accession
Sequence
>LE_HBa0145I04_SP6_71430 (raw untrimmed)
GGTAAAAAGGTCTGTTCGTCTCCTCAGTGATAGCAACGAAGTACTCATACTTTTCTGTGACACCAATTAGAATCTTTTCCACAACTTGTTGGTCAGAAAT
TGTATCACTATGATTTCTCATTTCATTAACAATATTCATGACTCTTGTGCAATATTCATCAATTTTTTCAGATTCTATCATCTTTAAATTTTGAAACTCT
CTTCTAAGAGTTTGAAGATTTATAGTGCGTACCTTTTCGTCACCATACACCACAGTTTCCAGAAAATCCAAAGCTTCCTTTGCAGTCTCACAAGTAGCAA
TTTTTGCAAAATATGCTCTTGAGACTCCCATTTGGATTTTGCTCAAGGCTTTTGCATCTTGATGATACTTAGCCTCAAGATTTTTCATCTCTGTCGCTAT
AAGATCACCATCGTTGTCTGGCTCTTCAAAGTCATTTGCAACAATAGTCCACAAACCTTCAGCTTTCAAATGTGTTCTCATTCTTATCTTCCAGTATTCA
AAATCAGTTCCATCAAAAAATGGAGTAAGAACAACTGAAGAATCAGCACCTTCATTTGTTTTGGATGCCATATTCTTTTCTTCACCTAACCCCAGTTTAA
GAACGAAAACCTGCTCTTGATACCAATTTGTAGGAAATATGAAAGGAAAAATAATATCTTTAGAAAATGCTCAAGTTTATTATAGGCTACTTAAGGCCAC
TTAAAATGATTACAATCATCAACTACATCACTATTTATACCTACTCAAAAAAGAAAACAAAAAATCTTGCACAAATTAACTAAATACATGTATCACAATT
TACCAAATACATGTACTACCAAAATCTA
>LE_HBa0145I04_SP6_71430 (trimmed for quality and cloning vector)
TCTCCTCAGTGATAGCAACGAAGTACTCATACTTTTCTGTGACACCAATTAGAATCTTTTCCACAACTTGTTGGTCAGAAATTGTATCACTATGATTTCT
CATTTCATTAACAATATTCATGACTCTTGTGCAATATTCATCAATTTTTTCAGATTCTATCATCTTTAAATTTTGAAACTCTCTTCTAAGAGTTTGAAGA
TTTATAGTGCGTACCTTTTCGTCACCATACACCACAGTTTCCAGAAAATCCAAAGCTTCCTTTGCAGTCTCACAAGTAGCAATTTTTGCAAAATATGCTC
TTGAGACTCCCATTTGGATTTTGCTCAAGGCTTTTGCATCTTGATGATACTTAGCCTCAAGATTTTTCATCTCTGTCGCTATAAGATCACCATCGTTGTC
TGGCTCTTCAAAGTCATTTGCAACAATAGTCCACAAACCTTCAGCTTTCAAATGTGTTCTCATTCTTATCTTCCAGTATTCAAAATCAGTTCCATCAAAA
AATGGAGTAAGAACAACTGAAGAATCAGCACCTTCATTTGTTTTGGATGCCATATTCTTTTCTTCACCTAACCCCAGTTTAAGAACGAAAACCTGCTCTT
GATACCAATTTGTAGGAAATATGAAAGGAAAAATAATATCTTTAGAAAATGCTCAAGTTTATTATAGGCTACTTAAGGCCACTTAAAATGATTACAATCA
TCAACTACATC

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.935286
Expected error %
0.137729
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAP46257.1| Score 156 E Value 6e-37 Identity 16.10% Align. Length 509bp (71.59%) Frame 3
putative polyprotein [Oryza sativa (japonica cultivar-group)] ref|XP_470160.1| putative polyprotein [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAP51797.1| Score 153 E Value 5e-36 Identity 15.90% Align. Length 509bp (71.59%) Frame 3
putative copia-type polyprotein [Oryza sativa (japonica cultivar-group)] ref|NP_919510.1| putative copia-type polyprotein [Oryza sativa (japonica cultivar-group)] gb|AAL75752.1| Putative copia-type polyprotein [Oryza sativa]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0145I04
Clone
Clone name
hba-145i4
LE_HBa0145I04
C12HBa0145I04
P145I04
Clone type
BAC
Contamination
none
Estimated length
90,334
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found