Genomic Clone End Sequence - Sol Genomics Network

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Clone Read LE_HBa0075N03_T7_85493

Sequence Read

Read Class

BAC ends

Read ID

LE_HBa0075N03_T7_85493

Primer

ID from Seq. Facility

LE_HBa_075_N03-T7

Chromatogram

[download]

Sequence

Version

1 (current)

Length (bp)

276 trimmed for vector and quality, 778 raw

Sequence

Show Raw Sequence

>LE_HBa0075N03_T7_85493 (trimmed for quality and cloning vector)
TGGAAAATCAATTCCATAAGGCAAATAATCAGCTCTAGCCAATGACCTAATCACATTATTATTTCCATTATCCACTAATGAATCACCAAATATAAAGTAA
CAAGGCACTTGTGGTTCTCCTTTTGCACTATAATTATCTCCTAATAAATTCAAAATTAATAAAAACACACACATTATCTTTAAATCTCCACACATCATGA
ATCCTTCCAAAAATAAAAAAAAAATAAAAATTGCGAGAAAGGAAAAGATGGAAGACAATATATGTATATGGTTTAT

Warnings:

Not screened for repetitive elements
Not screened for chimera

Quality Report
Processed by SGN	Vector signature flanking vector not found in read	Quality trim threshold 20.5	Sequence entropy 0.89664	Expected error % 0.637908

Sequence Annotations

Automatic Annotations

Arabidopsis proteins (TAIR10) (ath/ATH_pep)

NCBI BLAST

At1g29660.1

Score 87 E Value 1e-18 Identity 22.20% Align. Length 179bp (64.86%) Frame 2

68414.m03625 GDSL-motif lipase/hydrolase family protein low similarity to family II lipase EXL1 [Arabidopsis thaliana] GI:15054382; contains InterPro Entry IPR001087 Lipolytic enzyme, G-D-S-L family

Arabidopsis proteins (TAIR10) (ath/ATH_pep)

NCBI BLAST

At5g45670.1

Score 74 E Value 1e-14 Identity 26.00% Align. Length 122bp (44.20%) Frame 2

68418.m05615 GDSL-motif lipase/hydrolase family protein similar to family II lipases EXL3 GI:15054386, EXL1 GI:15054382, EXL2 GI:15054384 from [Arabidopsis thaliana]; contains Pfam profile PF00657: GDSL-like Lipase/Acylhydrolase

NCBI NR non-redundant protein sequence database (genbank/nr)

NCBI BLAST

gb|AAM65973.1|

Score 87 E Value 1e-16 Identity 22.20% Align. Length 179bp (64.86%) Frame 2

lipase/hydrolase, putative [Arabidopsis thaliana]

NCBI NR non-redundant protein sequence database (genbank/nr)

NCBI BLAST

gb|AAM91390.1|

Score 87 E Value 1e-16 Identity 22.20% Align. Length 179bp (64.86%) Frame 2

At1g29660/F15D2_21 [Arabidopsis thaliana] ref|NP_174259.1| GDSL-motif lipase/hydrolase family protein [Arabidopsis thaliana] gb|AAK91429.1| At1g29660/F15D2_21 [Arabidopsis thaliana] gb|AAG51756.1| lipase/hydrolase, putative; 114382-116051 [Arabidopsis thaliana] pir||H86419 probable lipase/hydrolase, 114382-116051 [imported] - Arabidopsis thaliana

Manual Annotations

No manual annotations found.

BAC

Clone LE_HBa0075N03

Clone
Clone name hba-75n3 LE_HBa0075N03 P075N03
Clone type BAC
Contamination none
Estimated length not yet estimated

Library
Name Tomato HindIII BAC Library	Cloning host E. coli
Organism Solanum lycopersicum (formerly Lycopersicon esculentum)	Cloning vector pBeloBAC11
Accession Heinz 1706	Restriction enzyme HindIII

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