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SC Spread ID	Images			Distance from centromere (percentage of arm length)
247-09 1	Composite	Fluorescence	Phase	94.1%
247-13 1	Composite	Fluorescence	Phase	100.0%
247-15 1	Composite	Fluorescence	Phase	99.1%
247-22 1	Composite	Fluorescence	Phase	97.4%
247-26 1	Composite	Fluorescence	Phase	93.4%
247-28 1	Composite	Fluorescence	Phase	97.0%
247-30 1	Composite	Fluorescence	Phase	100.0%
247-32 1	Composite	Fluorescence	Phase	98.4%
247-33 1	Composite	Fluorescence	Phase	98.3%
247-35 1	Composite	Fluorescence	Phase	100.0%
247-38 1	Composite	Fluorescence	Phase	97.0%
247-40 1	Composite	Fluorescence	Phase	95.4%
247-42 1	Composite	Fluorescence	Phase	100.0%
247-43 1	Composite	Fluorescence	Phase	100.0%
Mean±sd				97.9%±2.3%

Given an estimated length of 7.3µm for chromosome 12Q and the mean percentage above, this BAC is approximately 7.1µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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