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SC Spread ID	Images			Distance from centromere (percentage of arm length)
239-13 1	Composite	Fluorescence	Phase	66.5%
239-14 1	Composite	Fluorescence	Phase	67.3%
239-15 1	Composite	Fluorescence	Phase	65.9%
239-17 1	Composite	Fluorescence	Phase	65.9%
239-20 1	Composite	Fluorescence	Phase	69.8%
239-22 1	Composite	Fluorescence	Phase	64.4%
239-23 1	Composite	Fluorescence	Phase	68.9%
239-25 1	Composite	Fluorescence	Phase	70.7%
239-27 1	Composite	Fluorescence	Phase	68.1%
239-28 1	Composite	Fluorescence	Phase	68.3%
239-29 1	Composite	Fluorescence	Phase	66.8%
239-31 1	Composite	Fluorescence	Phase	70.6%
239-36 1	Composite	Fluorescence	Phase	64.4%
239-38 1	Composite	Fluorescence	Phase	65.7%
Mean±sd				67.4%±2.1%

Given an estimated length of 7.4µm for chromosome 5P and the mean percentage above, this BAC is approximately 5.0µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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