Analysis of gene families encoding acidic and basic beta-1,3-glucanases of tobacco.

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TitleAnalysis of gene families encoding acidic and basic beta-1,3-glucanases of tobacco.
Series nameProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue22
Year1990
Pages8756-60
AuthorsLinthorst, HJ. Melchers, LS. Mayer, A. van Roekel, JS. Cornelissen, BJ. Bol, JF
AbstractHealthy tobacco plants accumulate beta-1,3-glucanases (glucan endo-1,3-beta-glucosidase; EC 3.2.1.39) in their roots and in specific parts of the flowers. After infection with tobacco mosaic virus, acidic and basic beta-1,3-glucanases are induced in the inoculated and virus-free leaves of the plant. An analysis of cDNA clones demonstrated that at least five genes for acidic beta-1,3-glucanases are induced after tobacco mosaic virus infection. Southern blot analysis indicated that the tobacco genome contains approximately eight genes for acidic beta-1,3-glucanases and a smaller number of genes encoding basic beta-1,3-glucanases. Genes from both gene families were cloned and sequenced. The basic isozymes contain a C-terminal extension that is cleaved off during their targeting to the vacuoles. This extension is absent in the acidic isozymes, which accumulate extracellularly. Northern blot hybridization showed that genes encoding acidic and basic beta-1,3-glucanases are strongly induced after tobacco mosaic virus infection or salicylate treatment of tobacco. The cloning of these genes is a first step toward the identification of regulatory elements involved in their coordinate induction.
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