Co-dominant SCAR markers for detection of the Ty-3 and Ty-3a loci from Solanum chilense at 25 cM of chromosome 6 of tomato.
| Publication details |
| Title | Co-dominant SCAR markers for detection of the Ty-3 and Ty-3a loci from Solanum chilense at 25 cM of chromosome 6 of tomato. |
| Series name | Tomato Genetics Coop. Rept. |
| Volume | 57 |
| Issue | |
| Year | 2007 |
| Pages | 25-28 |
| Authors | Ji, Y.. Salus, M.S.. van Betteray, B.. Smeets, J.. Jensen, K.S.. Martin, C.T.. Mejia, L.. Scott, J.W.. Havey, M.J.. Maxwell, D.P. |
| Abstract | Co-dominant SCAR marker, P6-25F2R5, linked to begomovirus resistance introgressions derived from S. chilense on chromosome 6 at 25 cM are described. These PCR primers amply three different size fragments corresponding to ty-3 (no introgression), Ty-3 (introgression for S. chilense LA2779) and Ty-3a (introgression from S. chilense LA1932). These introgressions for the Ty-3 locus mapped to a region that includes the FER locus (25 cM, BAC clone 56B23, AY678298). PCR reactions were completed at an annealing temperature of 53 C with forward primer, P6-25-F2, 5’ GGT AGT GGA AAT GAT GCT GCT C, and reverse primer, P6-25-R5, 5’ GCT CTG CCT ATT GTC CCA TAT ATA ACC. |
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