Expression of Atropa belladonna putrescine N-methyltransferase gene in root pericycle.
| Publication details |
| Title | Expression of Atropa belladonna putrescine N-methyltransferase gene in root pericycle. |
| Series name | Plant & cell physiology |
| Volume | 40 |
| Issue | 3 |
| Year | 1999 |
| Pages | 289-97 |
| Authors | Suzuki, K. Yamada, Y. Hashimoto, T |
| Abstract | The cDNAs encoding putrescine N-methyltransferase (PMT), which catalyzes the S-adenosylmethionine-dependent N-methylation of putrescine at the first committed step in the biosynthetic pathways of tropane alkaloids, were isolated from Atropa belladonna and Hyoscyamus niger. These PMTs, however, lacked the N-terminal tandem repeat arrays previously found in Nicotiana PMTs. AbPMT1 RNA was much more abundant in the root of A. belladonna than was AbPMT2 RNA. The 5'-flanking region of the AbPMT1 gene was fused to the beta-glucuronidase (GUS) reporter gene and transferred to A. belladonna. Histochemical analysis showed that GUS is expressed specifically in root pericycle cells and that the 0.3-kb 5'-upstream region was sufficient for pericycle-specific expression. Treatment of A. belladonna roots with methyl jasmonate did not up-regulate the expression of GUS or endogenous AbPMT genes. The regulation of tropane alkaloid biosynthesis is discussed and compared with that of nicotine biosynthesis. |
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