Sol Genomics Network

SGN Marker C2_At2g01110

SGN-M3927
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	310764 (SGN) 125518 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U202030	-	-	-
Lycopersicon Combined	Single	SGN-U220240	-	-	-
Solanum tuberosum	Single	SGN-U263209	-	-	-
Arabidopsis	Single	At2g01110 on TAIR	-	-	-

Mapped locations

Map: Tomato - Kazusa and SolCAP markers mapped to genome
Map version 105 (location: 49224)
Chromosome 5
Position 2.86 MB
Confidence uncalculated
Protocol CAPS

Tomato - Kazusa and SolCAP markers mapped to genome

Map: Kazusa F2-2000 genetic map
Map version 103 (location: 47155)
Chromosome 5
Position 38.00 cM
Confidence uncalculated
Protocol unknown

Kazusa F2-2000 genetic map

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46623)
Chromosome 10
Position 86.90 cM
Confidence F(LOD3)
Protocol CAPS

Tobacco N. tomentosiformis

Map: Pepper-COSII
Map version 58 (location: 41364)
Chromosome 11
Position 0.00 cM
Confidence F(LOD3)
Protocol CAPS

Pepper-COSII

PCR data Exp. ID 43340

Forward primer (5'-3')

ATTTGTGCTCGTACTCATGTTCAG

Accessions and product sizes

Approximate temperature

60°C

Reverse primer (5'-3')

TCCAAATCGACAGCATTTGATCTC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38283)
Chromosome 5
Position 37.00 cM
Confidence F(LOD3)
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39549

Forward primer (5'-3')

ATTTGTGCTCGTACTCATGTTCAG

Accessions and product sizes

Solanum pennellii TA56

650

Solanum lycopersicum TA209

650

Approximate temperature

55°C

Reverse primer (5'-3')

TCCAAATCGACAGCATTTGATCTC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

400

Solanum pennellii TA56

500

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25549)
Chromosome 2
Position 0.08 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10494
Forward primer (5'-3') ATTTGTGCTCGTACTCATGTTCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAAATCGACAGCATTTGATCTC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 650	Approximate temperature 55°C

PCR data Exp. ID 10495
Forward primer (5'-3') ATTTGTGCTCGTACTCATGTTCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAAATCGACAGCATTTGATCTC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 650	Approximate temperature 55°C

PCR data Exp. ID 10496
Forward primer (5'-3') ATTTGTGCTCGTACTCATGTTCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAAATCGACAGCATTTGATCTC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 650	Approximate temperature 55°C

PCR data Exp. ID 12891
Forward primer (5'-3') ATTTGTGCTCGTACTCATGTTCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAAATCGACAGCATTTGATCTC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 720	Approximate temperature 55°C

PCR data Exp. ID 12892
Forward primer (5'-3') ATTTGTGCTCGTACTCATGTTCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAAATCGACAGCATTTGATCTC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 720	Approximate temperature 55°C

PCR data Exp. ID 12893
Forward primer (5'-3') ATTTGTGCTCGTACTCATGTTCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAAATCGACAGCATTTGATCTC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 720	Approximate temperature 55°C

PCR data Exp. ID 12894
Forward primer (5'-3') ATTTGTGCTCGTACTCATGTTCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAAATCGACAGCATTTGATCTC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 720	Approximate temperature 55°C

PCR data Exp. ID 12895
Forward primer (5'-3') ATTTGTGCTCGTACTCATGTTCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAAATCGACAGCATTTGATCTC	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 720	Approximate temperature 55°C

PCR data Exp. ID 12896
Forward primer (5'-3') ATTTGTGCTCGTACTCATGTTCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAAATCGACAGCATTTGATCTC	Enzymes
Accessions and product sizes Physalis TA1367: 600	Approximate temperature 55°C

PCR data Exp. ID 12897
Forward primer (5'-3') ATTTGTGCTCGTACTCATGTTCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAAATCGACAGCATTTGATCTC	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 480	Approximate temperature 55°C

PCR data Exp. ID 12898
Forward primer (5'-3') ATTTGTGCTCGTACTCATGTTCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAAATCGACAGCATTTGATCTC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 480	Approximate temperature 55°C

PCR data Exp. ID 32616
Forward primer (5'-3') ATTTGTGCTCGTACTCATGTTCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAAATCGACAGCATTTGATCTC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 720	Approximate temperature 55°C

PCR data Exp. ID 32886
Forward primer (5'-3') ATTTGTGCTCGTACTCATGTTCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAAATCGACAGCATTTGATCTC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 720	Approximate temperature 55°C

PCR data Exp. ID 32887
Forward primer (5'-3') ATTTGTGCTCGTACTCATGTTCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAAATCGACAGCATTTGATCTC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 720	Approximate temperature 55°C

PCR data Exp. ID 32888
Forward primer (5'-3') ATTTGTGCTCGTACTCATGTTCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAAATCGACAGCATTTGATCTC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 720	Approximate temperature 55°C

PCR data Exp. ID 32889
Forward primer (5'-3') ATTTGTGCTCGTACTCATGTTCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAAATCGACAGCATTTGATCTC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 720	Approximate temperature 55°C

PCR data Exp. ID 10497
Forward primer (5'-3') ATTTGTGCTCGTACTCATGTTCAG	Digested band sizes (using HaeIII) Solanum lycopersicum LA925: 400 Solanum pennellii LA716: 500	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAAATCGACAGCATTTGATCTC	Enzymes All possible enzymes for pennellii and and lycopersicum: HaeIII
Accessions and product sizes Solanum lycopersicum LA925: 650 Solanum pennellii LA716: 650	Approximate temperature 55°C

Unigene blast matches for primers

SGN-U220240
SGN-U294191

Overgo hybridization and physical mapping

C2_At2g01110 was used as an overgo probe on plate 13 [well C2]

Plausible BAC Matches: P255M10, P282K01, P309L13, P251J14, P313F03, P294K10, P055G23, P237E15, P271P05, P081N16, P041C18, P006L06, P237P14, P244P17

Overgo Sequences

A sequence

CTTTACTTGGGTGGAGCATGGATG

B sequence

GCCCAGAAAGCTTAACCATCCATG

AB sequence

CTTTACTTGGGTGGAGCATGGATGGTTAAG

Marker sequence

ATGGGAAGTTCAAGTGCTCTCATCTCAAATCTTCACCTGACTCAAACTAA
CTGTTTCAAATGCTTGAATTCAAGAACTTCTCTGAATATCAACCCCACAA
GGCCAAAATTGAATCTTTCTAGCAGAAAATGCATCAAAAAGTTCAGCAGA
CTTGTTTGTTCTGCTGTTGAAGATTCCATGGAGAAACAGAGAGAAATTAG
TGGAGCAAATGCTAGCAGTCTTGGTTCAGCTGTTGAAGATAGACCTGATG
TCGGTGATGGCTCAAGCAAGAGTTTATTTAAGAACGNNNNNGTGGATCAG
ATAGTGAAGGGAATGTTGTTTATGATTTCCTTTACCCTAATAAAGAGCTG
CTACCAGATGATAAAGAAATGACTCTATTTGATCATTTGGAGGAGTTGCG
CCAGAGACTCTTTGTGTCTGTTTTGGCCGTTGGTGCTGCTATAGTGGGAT
GTTTTGCCTTCTCAAAAGAACTTATCTTGATTCTTGAAGCCCCTGTTCTA
GCACAAGGTGTTCGATTTTTGCAACTAGGTCCAGGAGAGTTCTTTTTCAC
TACTTTAAAANNNNNGTCTCAGGATATAGTGGCCTTCTCTTAGGAGCTCC
TGTGATTCTCTACGAGATCATAGCCTTCGTTCTTCCTGGTTTGACAATGT
CAGAAAGAAGATTCCTGGCACCAATTGTCCTTGGATCCTCTGTTCTTTTC
TATGCCGGCATTGTTTTCTCACACTTGGTCCTCACTCCAGCAGCCTTGAA
TTTCTTTGTTAATTATGCAGAAGGGGCTGTGGAATCTTTTTGGTCCATTG
ATCAATACTTCGAATTTGTGCTCGTACTCATGTTCAGTACAGGGTTGTCT
TTCCAGNNNNNGTTCCTGTCATTCAACTGCTTCTGGGACAAACTGGTCTT
GTGTCAGGAGATCAAATGCTGTCGATTTGGAGATACGTGGTGGTAGGTGC
TGTCGTTGCTGCTGCAGTGCTCACACCATCAACTGATCCCCTTACTCAAA
TGCTTCTAGCTGGCCCACTTTTAGGTCTTTACTTGGGTGGAGCATGGATG
GTTAAGCTTTCTGGGCGATAA

Universal primers for Asterid species

No additional PCR data found.

Other COSII sequence data

BLASTX result of original unigene sequences against Arabidopsis protein database

BLASTX result of original unigene sequences against Arabidopsis protein database

Related Markers

Related Genotyped Markers: These markers have been genotyped and share a name with this marker

Genomic location of C2_At2g01110

%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D3927 marker 3927

User comments

Please wait, checking for comments. (If comments do not show up, access them here)

Accessions	Seedlots
Accessions		Breeding Program	Seedlot Name	Contents	Seedlot Location	Count	Weight(g)

Attribute	Value
Project Name:
Start Date:
End Date:
Event Type:
Event Description:
Event Web URL:

SGN is supported by , and hosted at
Cite SGN using | Disclaimer