Clone hba-20a12

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Clone
Clone name
hba-20a12
LE_HBa0020A12
C10HBa0020A12
P020A12
Clone type
BAC
Contamination
none
Estimated length
101,348
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
not present in Tomato FPC (AGI 2005)
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
T0743Tomato-EXPEN 2000plate 16H4
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 10
FISH map 
View on FISH map chromosome 10
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
470-02 1CompositeFluorescencePhase61.6%
470-03 1CompositeFluorescencePhase61.3%
470-04 1CompositeFluorescencePhase66.5%
470-08 1CompositeFluorescencePhase62.7%
470-09 1CompositeFluorescencePhase61.2%
470-11 1CompositeFluorescencePhase64.7%
470-13 1CompositeFluorescencePhase63.2%
470-15 1CompositeFluorescencePhase61.9%
470-16 1CompositeFluorescencePhase62.4%
470-17 1CompositeFluorescencePhase64.3%
470-22 1CompositeFluorescencePhase64.0%
470-23 1CompositeFluorescencePhase64.7%
470-25 1CompositeFluorescencePhase62.9%
470-26a 1CompositeFluorescencePhase61.3%
470-26b 1CompositeFluorescencePhase62.2%
470-28 1CompositeFluorescencePhase61.6%
470-30 1CompositeFluorescencePhase64.6%
470-31 1CompositeFluorescencePhase65.4%
470-35 1CompositeFluorescencePhase63.9%
470-36 1CompositeFluorescencePhase62.4%
470-37 1CompositeFluorescencePhase61.1%
470-41 1CompositeFluorescencePhase63.8%
470-42 1CompositeFluorescencePhase62.1%
470-44 1CompositeFluorescencePhase63.9%
470-49 1CompositeFluorescencePhase65.5%
470-50 1CompositeFluorescencePhase62.8%
470-52 1CompositeFluorescencePhase61.8%
470-53 1CompositeFluorescencePhase60.7%
470-54 1CompositeFluorescencePhase66.5%
correction 280.1%
Mean±sd63.7%±1.6%

Given an estimated length of 10.1µm for chromosome 10Q and the mean percentage above, this BAC is approximately 6.4µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

2The position of this BAC has been adjusted slightly to reflect its location relative to the typical position of marker BAC LE_HBa0234C10.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 10 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 10
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
Uploaded by account 'manual'
Full Sequence
135,014 bp
C10HBa0020A12.1
AC238917.3 (GenBank) 		[View]
[Download fasta]
[Download full submission] (0.09 MB)
End Sequences
Warnings
  • poor correspondence with in vitro restriction fragments
    (match score below 0.7)
 FTP
Chromosome 10 Sequencing repository (FTP)
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (16)
2322, 2469, 2955, 3058, 3398, 3509, 3544, 5018, 5018, 5320, 5934, 6506, 6979, 10872, 14879, 19567
HindIII in silico (33)
1014, 1295, 1606, 1784, 1789, 1935, 2120, 2202, 2212, 2235, 2351, 2415, 2698, 2927, 3142, 3293, 3670, 3700, 3870, 3898, 3975, 4630, 4791, 5084, 5134, 5513, 5534, 5743, 5899, 6510, 6847, 6852, 7543
Fragment Lengths Match Score
0.67
Annotations  
Browse
LE_HBa0020A12 has no browsable sequence annotations
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Seq. Center Reported Status
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 and  bp
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End Sequences

e.g. crystallin
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Map
Map position
 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

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