Clone hba-188b22

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Clone
Clone name
hba-188b22
LE_HBa0188B22
C12HBa0188B22
P188B22
Clone type
BAC
Contamination
none
Estimated length
110,707
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg2000 : [coherent], 10 members:
C07HBa0026P16, C12HBa0145I04, C12HBa0188B22, C12HBa0244L05, C12HBa0267O16, C12HBa0273I22, C12HBa0285M08, C12HBa0302K10, C12HBa0303O08, C12HBa0304P17
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
CT135Tomato-EXPEN 2000plate 6B7
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 12
FISH map 
View on FISH map chromosome 7
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
339-02 1CompositeFluorescencePhase29.5%
339-27 1CompositeFluorescencePhase31.1%
339-31 1CompositeFluorescencePhase27.3%
339-32 1CompositeFluorescencePhase29.3%
339-33 1CompositeFluorescencePhase28.8%
339-34 1CompositeFluorescencePhase30.3%
339-36 1CompositeFluorescencePhase30.1%
339-38 1CompositeFluorescencePhase26.7%
339-40 1CompositeFluorescencePhase32.5%
339-41 1CompositeFluorescencePhase33.1%
Mean±sd29.9%±2.0%

Given an estimated length of 10.8µm for chromosome 7Q and the mean percentage above, this BAC is approximately 3.2µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 12 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 12
HTGS phase
none
Sequencing Status
none
Sequencing Organization
not recorded
Full Sequence
Sequence not available.
End Sequences
Warnings
none
FTP
Chromosome 12 Sequencing repository (FTP)
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (30)
1004, 1004, 1300, 1442, 1526, 1593, 1593, 1843, 1880, 2184, 2198, 2251, 2919, 3116, 3271, 3271, 3383, 3408, 3513, 3567, 3815, 4131, 4131, 4522, 4779, 5649, 8390, 9392, 9726, 9906
Annotations  
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LE_HBa0188B22 has no browsable sequence annotations
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 and  bp
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e.g. AP009318.1 or AP009318
Sequencing on Chromosome
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Annotations
End Sequences

e.g. crystallin
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Map
Map position
 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

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